Genetically engineered mushroom created using CRISPR-cas9

Q.  First genetically engineered mushroom has been produced using which method that has also received USDA clearance?
- Published on 19 Apr 16

a. CRISPM-Cas10 genome editing tool
b. Talens
c. CRISPR-Cas9 gene editing tool
d. None of the above

ANSWER: CRISPR-Cas9 gene editing tool
 
A species of genetically engineered mushrooms has been freed of regulation by USDA and it can be sold and grown without prior permission. There are about 30 genetically modified organisms that have been freed by the USDA
  • This marks the first time USDA has given approval for tool CRISPR-Cas9 edited mushroom species
  • Known as the common white button mushroom, this fungus species (Agaricus bisporus) was genetically engineered by Yinong Yang of Pennsylvania State University using CRISPR-Cas9.
  • The engineered mushrooms do not brown easily when sliced. This can improve their shelf-life, thereby rendering them valuable commercially.
  • The browning of the mushroom is caused by the enzyme polyphenol oxidase (PPO) and scientists got rid of one of the six PPO genes which reduced the enzyme’s activity by 30 per cent.

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