Bio Engineering interview questions and answers

          

Bio Engineering interview questions and answers


Part 1   Part 2   Part 3   Part 4   Part 5   Part 6

What is therapeutic cloning?
Explain the mechanism of ELISA. What are its uses?
Explain microarrays. How are they related to DNA?
What is the principle behind DNA fingerprinting?
What do you understand by MRI?
What is BMI?

What is therapeutic cloning?

In the process of cloning DNA or a part of DNA is duplicated. This process is also called somatic cell nuclear transfer. In this process embryo is utilized for cloning. Embryo comprises of stem cells which is later employed in regeneration applications. The embryonic stem cells are renewable and are pluripotent.

Explain the mechanism of ELISA. What are its uses?

ELISA stands for enzyme linked immunosorbent assay. It is a wet lab type analytical biochemistry assay. It can detect presence of a substance in a liquid or wet sample by using one subtype of heterogeneous, solid-phase enzyme immunoassay. ELISA can be used as ligand binding assays. It is also used as a diagnostic tool in medicine and plant pathology. Quality check operations can also be performed in various industries with the help of ELISA.

Explain microarrays. How are they related to DNA?

Microarrays are matrix in the form of arrays where DNA oligonucleotides of DNA sequences are spotted. They can be used for gene expression profiling, single nucleotide polymorphism detection, detection of alternative splicing and for various other purposes. Microarrays have the capability to perform hybridization of coda with the help of probes. A microarray chip is capable to perform a large set of genetic related experiments concurrently.

What is the principle behind DNA fingerprinting?

DNA fingerprinting is the technique of genetic fingerprinting. In this technique, DNA sequence can be used for identification of an individual. The main application of DNA fingerprinting is forensics. The main principle behind behind DNA fingerprinting is Polymerase Chain Reaction. This technique is also popularly known as DNA profiling.

What do you understand by MRI?

MRI stands for Magnetic Resonance Imaging. This technique uses medical imaging that provides a detailed structure of internal organs; especially soft tissues.MRI provides good contrast between different soft tissues. A strong magnetic field is used in MRI which generates images and models of the specified organ. MRI works on the principle of nuclear magnetic resonance to generate image of nuclei of atoms inside the body.

What is BMI?

BMI stands for body mass index. This index represents the comparison of the person's height and weight. The weight of the person is divided by the square of the person’s height. The SI unit for measuring BMI in kg/sq.m. BMI should not be treated as a technique for measuring body fat.

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